酶 | 寡核苷酸序列 | 切割率% |
2 hr | 20 hr |
Not I | TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA | 0 10 10 25 25 | 0 10 10 90 >90 |
Nsi I | TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT | 10 >90 | >90 >90 |
Pac I | TTAATTAA GTTAATTAAC CCTTAATTAAGG | 0 0 0 | 0 25 >90 |
Pme I | GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG | 0 0 0 75 | 0 25 50 >90 |
Pst I | GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT | 0 10 >90 >90 0 | 0 10 >90 >90 0 |
Pvu I | CCGATCGG ATCGATCGAT TCGCGATCGCGA | 0 10 0 | 0 25 10 |
Sac I | CGAGCTCG | 10 | 10 |
Sac II | GCCGCGGC TCCCCGCGGGGA | 0 50 | 0 >90 |
Sal I | GTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG * ^0 A# l* B- O, |: U
ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA | 0 10 10 | 0 50 75 |
Sca I | GAGTACTC AAAAGTACTTTT | 10 75 | 25 75 |
Sma I | CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA | 0 0 10 >90 | 10 10 50 >90 |
Spe I | GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG | 10 10 0 0 | >90 >90 50 50 |
Sph I | GGCATGCC CATGCATGCATG ACATGCATGCATGT | 0 0 10 | 0 25 50 |
Stu I | AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT | >90 >90 >90 | >90 >90 >90 |
Xba I | CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG | 0 >90 75 75 | 0 >90 >90 >90 |
Xho I | CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG | 0 10 10 | 0 25 75 |
Xma I | CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA | 0 25 50 >90 | 0 75 >90 >90 |
$ {! ?0 l2 ^. ?7 p4 c | 酶 | 寡核苷酸序列 | 切割率% | 6 S# z3 r- U' ?1 Q- g3 z' V7 h6 Z3 N
|
+ _: t0 ~% ^* X) Z& d | 2 hr | 20 hr |
# m* g( k; o1 Q0 F* s9 r |
2 Y7 V, N) k9 P7 Z4 o- h* R# {
| Acc I | GGTCGACC CGGTCGACCG CCGGTCGACCGG | 0 0 0 | 0 0 0 |
c# F/ \. [7 M |
) E5 X j; n4 m$ H
| Afl III | CACATGTG CCACATGTGG CCCACATGTGGG | 0 >90 >90 | 0 >90 >90 |
9 N! ]6 a* _8 j* v9 g$ D( ~ |
8 Z& P% N( ?7 Z' Y, t8 M0 ^$ ~$ L | Asc I | GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA | >90 >90 >90 | >90 >90 >90 | * a' ^" e( O; J9 b: I* N
|
& X9 ]& A S( U/ N, [+ F- F
| Ava I | CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA | 50 >90 >90 | >90 >90 >90 |
; s% b- y# E/ C: k) [) q |
7 W" X9 R& B3 i, z4 j | BamH I | CGGATCCG CGGGATCCCG CGCGGATCCGCG | 10 >90 >90 | 25 >90 >90 | ; \3 K4 p! h/ @, C. w
|
) y0 f$ R& N6 W% S$ Y/ K | Bgl II | CAGATCTG GAAGATCTTC GGAAGATCTTCC | 0 75 25 | 0 >90 >90 |
+ V4 T1 Q# b) P7 v( U! s+ f |
* G# a2 P; A9 T) z" Q* B | BssH II | GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA | 0 0 50 | 0 0 >90 | 0 L# D2 a: k- L9 m( n8 |) ]: W
|
& v: @& g" B$ [6 C, k8 i& B
| BstE II | GGGT(A/T)ACCC | 0 | 10 |
. ^4 ?% j; n/ x% c4 i, D |
0 X* i0 Y. U! o$ H/ B- Y | BstX I | AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT | 0 25 25 | 0 50 >90 |
" i4 S" E$ K+ p |
$ U* K2 G6 |$ ]$ S
| Cla I | CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG | 0 0 >90 50 | 0 0 >90 50 | 1 E. [7 O7 P0 E! }# I
|
6 K6 f- F: d( P( W- \
| EcoR I | GGAATTCC CGGAATTCCG CCGGAATTCCGG | >90 >90 >90 | >90 >90 >90 |
" F4 D2 `6 o9 L |
; P4 R% m8 T3 h, M
| Hae III | GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA | >90 >90 >90 | >90 >90 >90 |
9 I7 E/ d! p- k+ }9 V$ V! e |
# q% y# Y5 ^6 }( I
| Hind III | CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG | 0 0 10 | 0 0 75 | C6 g' T0 U# d' A& I) h6 x
|
+ I+ F0 I2 U) P0 F3 f | Kpn I | GGGTACCC GGGGTACCCC CGGGGTACCCCG | 0 >90 >90 | 0 >90 >90 | $ U$ S! r+ x7 w3 k% ]/ S( h! J
|
6 `: O, X) L( i8 Z4 i5 Q | Mlu I | GACGCGTC CGACGCGTCG | 0 25 | 0 50 | U: g- i/ D$ B/ m5 c1 M9 n
|
8 Z4 j" m2 |0 K: Z# i F | Nco I | CCCATGGG CATGCCATGGCATG | 0 50 | 0 75 | 7 ^9 g3 g: i2 M& o) B- M$ g5 U* ~/ ~1 A
|
: a: e- W6 o r0 y) p
| Nde I | CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC | 0 0 0 0 75 75 | 0 0 0 0 >90 >90 | % I6 S4 B. _6 ]/ M3 Z6 y" ]6 H
|
7 n3 E8 _" H) [1 k1 T' ]
| Nhe I | GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG | 0 10 10 | 0 25 50 | ! X; @0 Q f* c
|
