氨基酸及其密码子对照

Alanine	Ala	A	GCU, GCC, GCA, GCG
Arginine	Arg	R	CGU, CGC, CGA, CGG, AGA, AGG
Asparagine	Asn	N	AAU, AAC
Aspartic acid	Asp	D	GAU, GAC
Cysteine	Cys	C	UGU, UGC
Glutamine	Gln	Q	CAA, CAG
Glutamic acid	Glu	E	GAA, GAG
Glycine	Gly	G	GGU, GGC, GGA, GGG
Histidine	His	H	CAU, CAC
Isoleucine	Ile	I	AUU, AUC, AUA
Leucine	Leu	L	UUA, UUG, CUU, CUC, CUA, CUG
Lysine	Lys	K	AAA, AAG
Methionine	Met	M	AUG
Phenylalanine	Phe	F	UUU, UUC
Proline	Pro	P	CCU, CCC, CCA, CCG
Serine	Ser	S	UCU, UCC, UCA, UCG, AGU,AGC
Threonine	Thr	T	ACU, ACC, ACA, ACG
Tryptophan	Trp	W	UGG
Tyrosine	Tyr	Y	UAU, UAC
Valine	Val	V	GUU, GUC, GUA, GUG
Start			AUG *
Stop			UAG (amber), UGA (opal), UAA (ochre)

AUG is the most common start codon. Alternative start codons include CUG in eukaryotes and GUG in prokaryotes.

 

单个碱基及其字符对照

A	Adenine
C	Cytosine
G	Guanine
T	Thymine
U	Uracil (RNA)
B	C, G, or T
D	A, G, or T
H	A, C, or T
K	G or T
M	A or C
N	A, T, C, or G
R	A or G
S	C or G
V	A, C, or G
W	A or T
Y	C or T

 

 

常用抗原决定基标记

Common Epitope Tags

Tag	Protein Sequence	DNA Sequence
FLAG	DYKDDDDK	GAC TAC AAA GAC GAT GAC GAC AAG
HA	YPYDVPDYA	TAC CCA TAC GAT GTT CCA GAT TAC GCT
His	HHHHHH	CAC CAC CAC CAC CAC CAC
Myc	EQKLISEEDL	GAA CAA AAA CTC ATC TCA GAA GAG GAT CTG

Due to the degenerate nature of the genetic code, the given sequence is one of a number of sequences that can encode the same epitope tag.

 

常用限制性酶及其酶切位点

AatII	GACGT C
AflII	C TTAAG
AgeI	A CCGGT
ApaI	GGGCC C
ApaLI	G TGCAC
AseI	AT TAAT
BamHI	G GATCC
BclI	T GATCA
BglII	A GATCT
BstBI	TT CGAA
ClaI	AT CGAT
DraI	TTT AAA
EagI	C GGCCG
EcoRI	G AATTC
EcoRV	GAT ATC
FspI	TGC GCA
HindIII	A AGCTT
HpaI	GTT AAC
KpnI	GGTAC C
NarI	GG CGCC
NcoI	C CATGG
NdeI	CA TATG
NheI	G CTAGC
NotI	GC GGCCGC
NruI