通用引物、常用引物及序列对照
General Primers
BGHrev | TAGAAGGCACAGTCGAGG |
GFPfusionREV | CTCCACTGACAGAAAATTTG |
M13 (-21) Forward | TGTAAAACGACGGCCAGT |
M13 (-40) | GTTTTCCCAGTCACGAC |
M13 Reverse | CAGGAAACAGCTATGAC |
MSCV-rev | CAGCGGGGCTGCTAAAGCGCATGC |
pBluescriptSK | TCTAGAACTAGTGGATC |
pBluescriptKS | TCGAGGTCGACGGTATC |
pGEX3' | CCGGGAGCTGCATGTGTCAGAGG |
pGEX5' | GGGCTGGCAAGCCACGTTTGGTG |
SP6 | ATTTAGGTGACACTATAG |
T7 Terminal | GCTAGTTATTGCTCAGCGG |
Universal T3 | GCAATTAACCCTCACTAAAGG |
Universal T7 | TAATACGACTCACTATAGGG |
Primers from Invitrogen
3'AOX1 | GCAAATGGCATTCTGACATCC |
5'AOX1 | GACTGGTCCAATTGACAAGC |
AC5 | ACACAAAGCCGCTCCATCAG |
AUG1 Forward | CAATTTACATCTTTATTTATTAACG |
AUG1 Reverse | GAAGAGAAAAACATTAGTTGGC |
BGH Reverse | TAGAAGGCACAGTCGAGG |
Baculovirus Forward | TTTACTGTTTTCGTAACAGTTTTG |
CMV Forward | CGCAAATGGGCGGTAGGCGTG |
CYC1 | GCGTGAATGTAAGCGTGAC |
EBV Reverse | GTGGTTTGTCCAAACTCATC |
EF-1a Forward | TCAAGCCTCAGACAGTGGTTC |
Ecdysone Forward | CTCTGAATACTTTCAACAAGTTAC |
GAL1 | AATATACCTCTATACTTTAACGTC |
M13/pUC Forward | CCCAGTCACGACGTTGTAAAACG |
M13/pUC Reverse | AGCGGATAACAATTTCACACAGG |
Metallothionein | CATCTCAGTGCAACTAAA |
OpIE2 Forward | CGCAACGATCTGGTAAACAC |
V5 Reverse | ACCGAGGAGAGGGTTAGGAT |
Xpress Forward | TATGGCTAGCATGACTGGT |
alpha-factor | TACTATTGCCAGCATTGCTGC |
cI Forward | GGATAGCCGGTCAGGTGTT |
pBAD Forward | ATGCCATAGCATTTTTATCC |
pBAD Reverse | GATTTAATCTGTATCAGG |
pCEP Forward | AGAGCTCGTTTAGTGAACCG |
pGAP | GTCCCTATTTCAATCAATTGAA |
pGENE Forward | CTGCTATTCTGCTCAACCT |
pHybLex Forward | AGGGCTGGCGGTTGGGGTTATTCGC |
pHybLex Reverse | GAGTCACTTTAAAATTTGTATACAC |
pJG4-5 Forward | GATGCCTCCTACCCTTATGATGTGCC |
pJG4-5 Reverse | GGAGACTTGACCAAACCTCTGGCG |
pREP Forward | GCTCGATACAATAAACGCC |
pRH Forward | CTGTCTCTATACTCCCCTATAG |
pRH Reverse | CAAAATTCAATAGTTACTATCGC |
pTrcHis Forward | GAGGTATATATTAATGTATCG |
pTrcHis Reverse | GATTTAATCTGTATCAGG |
pUni Forward | CTATCAACAGGTTGAACTG |
pUni Reverse | CAGTCGAGGCTGATAGCGAGCT |
pYESTrp Forward | GATGTTAACGATACCAGCC |
pYESTrp Reverse | GCGTGAATGTAAGCGTGAC |
Polyhedrin forward | AAATGATAACCATCTCGC |
Polyhedrin reverse | GTCCAAGTTTCCCTG |
Primers from BD Biosciences
3'AOX1 | GCAAATGGCATTCTGACATCC |
EGFP-C | CATGGTCCTGCTGGAGTTCGTG |
EGFP-N | CGTCGCCGTCCAGCTCGACCAG |
GAL1 | AATATACCTCTATACTTTAACGTC |
Gal4-AD | AATACCACTACAATGGAT |
HA | ATGTACCCATACCATGTTCCAGATTAC |
HAT | GAGGAGCACGCTCATGCCCAC |
LNCX | AGCTCGTTTAGTGAACCGTCAGATC |
MSCV | CCCTTGAACCTCCTCGTTCGACC |
Myc | GCATCAATGCAGAAGCTGATCTCA |
DsRed1-N | GTACTGGAACTGGGGGGACAG |
DsRed1-C | AGCTGGACATCACCTCCCACAACG |
pCMV | GATCCGGTACTAGAGGAACTGAAAAAC |
Other Primers
Bglob-intron-F | CTGGTCATCATCCTGCCTTT |
Bglob-pA-R | TTTTGGCAGAGGGAAAAAGA |
EXFP-R | GTCTTGTAGTTGCCGTCGTC (Golenbock lab) |
GFP-F | GGTCCTTCTTGAGTTTGTAAC |
GFP-R | CCATCTAATTCAACAAGAATTGGGACAAC (Ahmad lab) |
Gal10pro-F | GGTGGTAATGCCATGTAATATG (Stratagene) |
Gal4 N-term | GAGTAGTAACAAAGGTCAA |
GPDpro-F | CGGTAGGTATTGATTGTAATTCTG |
H1 | TCGCTATGTGTTCTGGGAAA |
HA-F | TACCCATACGACGTCCCAGA |
HA-R | TCTGGGACGTCGTATGGGTA |
hGH-PA-R | CCAGCTTGGTTCCCAATAGA |
IRES-R | CCTCACATTGCCAAAAGACG |
L4440 | AGCGAGTCAGTGAGCGAG (Caldwell lab) |
LacZ-R | GACAGTATCGGCCTCAGGAA |
LexA | CGTCAGCAGAGCTTCACCATTG (Caldwell lab) |
LKO.1 5' | GACTATCATATGCTTACCGT (Weinberg Lab) |
LucNrev | CCTTATGCAGTTGCTCTCC |
MBP-F | GATGAAGCCCTGAAAGACGCGCAG (Waugh lab) |
mPGK-F | CATTCTGCACGCTTCAAAAG |
MT1-F | GCTGTCCTCTAAGCGTCACC |
mU6-F | CAGCACAAAAGGAAACTCACC |
NOS-F | GCGTTCAAAAGTCGCCTAAG |
pACLU | AGAGCCTGATAAAAACGGTTAGCG (Joung lab) |
pBABE 3' | ACCCTAACTGACACACATTCC (Weinberg Lab) |
pBABE 5' | CTTTATCCAGCCCTCAC (Weinberg Lab) |
pBAC | CGCCAGGGTTTTCCCAGTCACGAC (Joung lab) |
pBMN 5' | GCTTGGATACACGCCGC |
pBRforBam | CTTGGAGCCACTATCGAC |
pBRrevBam | GGTGATGTCGGCGATATAGG |
pCasper-F | GGGTTTTATTAACTTACAT (Vosshall lab) |
pCAG-F | GCAACGTGCTGGTTATTGTG |
pcDL-F | GTTGCCTTTACTTCTAGGCCT (Kinet lab) |
pENTR-F | CTACAAACTCTTCCTGTTAGTTAG |
pGP | GGGGGATTTCTGTTCATGGGGG (Joung lab) |
pGP704-R | AACAAGCCAGGGATGTAACG |
pGV-2U | ACTAACATACGCTCTCCATCAA (Yamanaka lab) |
pLTet-F | ACTGAGCACATCAGCAGGAC |
pLXSN 5' | CCCTTGAACCTCCTCGTTCGACC (MSCV) |
pMT2-F | TTGCCTTTCTCTCCACAGGT |
pMX-S1811 | GACGGCATCGCAGCTTGGATACAC (Yamanaka lab) |
puro-F | GCAACCTCCCTTCTACGAGC |
RCAS-F | ACATGGGTGGTGGTATAGCGCTTGCG (Orsulic lab) |
RVprimer3 | CTAGCAAAATAGGCTGTCCC (Promega) |
SFFV-F | ATTGATTGACTGCCCACCTC |
SV40pA-R | GAAATTTGTGATGCTATTGC |
SV40pro-F | TATTTATGCAGAGGCCGAGG |
sv40-spliceR | CACAAAGATCCGGACCAAAG |
tac promoter | GAGCGGATAACAATTTCACACAGG (Waugh lab) |
TRC-F | CAAGGCTGTTAGAGAGATAATTGGA (Root lab) |
Ubx-F | AACTCGTACTTTGAACAGGC |
WPRE-R | CATAGCGTAAAAGGAGCAACA |
Information were obtained from Addgene.
欢迎大家跟贴补充！
