基因酷，您好！我是基因酷shangyb，我已认真阅读《资源保证协议》，并同意签署。我欲预存：
E.coli质粒（Novagen）：pET26b, pET42b, pETDuet-1
换取pAAV-MCS, pAAV-RC, pHelper三个质粒
我预共享的质粒基本信息：
1.载体名称：pET26b
   载体抗性：kana
   载体大小：5.3kb
   载体宿主：E. coli
2.载体名称：pET42b
   载体抗性：kana
   载体大小：5.9kb
   载体宿主：E. coli
3.载体名称：pETDuet-1
   载体抗性：amp
   载体大小：5.4kb
   载体宿主：E. coli

[ 本帖最后由 shangyb 于 08-10-8 15:35 编辑 ]

补充相关详细信息！
谢谢合作

补充信息：载体性质
1. pET26b
The pET-26b(+) vector (Cat. No. 69862-3) carries an N-terminal pelB signal sequence for
potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Unique sites
are shown on the circle map. Note that the sequence is numbered by the pBR322 convention,
so the T7 expression region is reversed on the circular map. The cloning/expression region of
the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented
so that infection with helper phage will produce virions containing single-stranded DNA that
corresponds to the coding strand. Therefore, single-stranded sequencing should be performed
using the T7 terminator primer
2. pET42b
The pET-42 series is designed for cloning and high-level expression of peptide sequences
fused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that the sequence
is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle
map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown
below. The f1 origin is oriented so that infection with helper phage will produce virions containing
single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing
should be performed using the T7 terminator primer (cat. no. 69337-3). Vector encoded sequence can
be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST
fusion protein with Factor Xa.
3. pETDuet-1
pETDuet™-1 is designed for the coexpression of two target genes. The vector contains
two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac
operator and a ribosome binding site (rbs). The vector also carries the pBR322-derived
ColE1 replicon, lacI gene and ampicillin resistance gene. This vector can be used in
combination with pACYCDuet™-1 (Cat. No. 71147-3) in an appropriate host strain for
the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced
using the pET Upstream Primer (Cat. No. 69214-3) and DuetDOWN1 Primer (Cat. No.
71179-3). Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat.
No. 71180-3) and T7 Terminator Primer

您好，我们有您需要的资源应助，您的资源也具备新颖性。先请您补充好相应的网站连接，谢谢

网址链接
1.pET26b
http://www.merckbiosciences.com/docs/docs/PROT/TB071.pdf
2.pET42b
http://www.merckbiosciences.com/docs/docs/PROT/TB240.pdf
3.pETDuet-1
http://www.merckbiosciences.com/docs/NDIS/TB337-000.pdf

shangyb酷友，您好！基因酷有资源可以应助，并确定您预共享的资源对于基因酷是“新颖的”，请您将以下信息站内短消息给我们：

一、个人信息包含内容如下：

您的真实姓名:
学习或工作单位:
联系电话（包括手机）:
QQ或MSN：
E-mail:
目前研究项目简介:（最少50字，主要说明项目的研究目的、难点和取得的结果。）
邮寄地址和邮编：
    以上信息主要是为了更加及时的给您邮寄资源，以及对共享资源的有效跟踪。基因酷对您的私人信息绝对保密，敬请酷友放心！（可以站内短信发给我们也可以Email：genecool@126.com
二、资源基本信息包含内容如下：

您对该资源的使用经历,主要包含该资源在您的实验室的使用频率;您的实验室最后一次使用该资源的日期，使用该资源的结果如何;如果可以希望您也能说明一下您使用该资源做过什么实验；
您获取该资源的途径；
对该资源您做过哪方面的验证工作，比如测序，表达，检测等；
    以上信息主要是为了配合保藏中心的鉴定工作。基因酷保证对这些信息的绝对保密，敬请您放心。

管理员，您好，您所要求填写的相关信息已发到genecool@126.com
中，请查收。

管理员，我已将相关信息发到您所给的邮箱中了，请问怎么还没有给我回复啊？

您好，我前天已经将邮寄地址用站内短信息发给您了！没有收到没？

已经收到了，谢谢。下午寄出质粒。