看很长时间没人更新，我只更新的部分，有心者继续更新，让LZ可以汇总，这样工作量就少些。

1.
总论
蛋白质相关实验技术操作全攻略
http://www.genecool.com/bbs/viewthread.php?tid=2444&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%9B%B8%E5%85%B3%E5%AE%9E%E9%AA%8C%E6%8A%80%E6%9C%AF%E6%93%8D%E4%BD%9C%E5%85%A8%E6%94%BB%E7%95%A5
蛋白质相关实验技术操作指南！
http://www.genecool.com/bbs/viewthread.php?tid=15&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%9B%B8%E5%85%B3%E5%AE%9E%E9%AA%8C%E6%8A%80%E6%9C%AF%E6%93%8D%E4%BD%9C%E6%8C%87%E5%8D%97
蛋白质相关实验技术操作指南（二）！
http://www.genecool.com/bbs/viewthread.php?tid=89&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%9B%B8%E5%85%B3%E5%AE%9E%E9%AA%8C%E6%8A%80%E6%9C%AF%E6%93%8D%E4%BD%9C%E6%8C%87%E5%8D%97
蛋白质分析路线、方法
http://www.genecool.com/bbs/viewthread.php?tid=1253&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E5%88%86%E6%9E%90%E8%B7%AF%E7%BA%BF%E3%80%81%E6%96%B9%E6%B3%95
蛋白质分析的相关技术介绍
http://www.genecool.com/bbs/viewthread.php?tid=420&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E5%88%86%E6%9E%90%E7%9A%84%E7%9B%B8%E5%85%B3%E6%8A%80%E6%9C%AF%E4%BB%8B%E7%BB%8D
（分享）核酸、蛋白技术参数资料、分子量标准及常用试剂的配制
http://www.genecool.com/bbs/viewthread.php?tid=3610&highlight=%EF%BC%88%E5%88%86%E4%BA%AB%EF%BC%89%E6%A0%B8%E9%85%B8%E3%80%81%E8%9B%8B%E7%99%BD%E6%8A%80%E6%9C%AF%E5%8F%82%E6%95%B0%E8%B5%84%E6%96%99%E3%80%81%E5%88%86%E5%AD%90%E9%87%8F%E6%A0%87%E5%87%86%E5%8F%8A%E5%B8%B8%E7%94%A8%E8%AF%95%E5%89%82%E7%9A%84%E9%85%8D%E5%88%B6  
一些与蛋白质方面有关的名词解析
http://www.genecool.com/bbs/viewthread.php?tid=1153&highlight=%E4%B8%80%E4%BA%9B%E4%B8%8E%E8%9B%8B%E7%99%BD%E8%B4%A8%E6%96%B9%E9%9D%A2%E6%9C%89%E5%85%B3%E7%9A%84%E5%90%8D%E8%AF%8D%E8%A7%A3%E6%9E%90

2.
蛋白质的提取：
【分享】蛋白质提取方法大全
http://www.genecool.com/bbs/viewthread.php?tid=5701&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E6%8F%90%E5%8F%96%E6%96%B9%E6%B3%95%E5%A4%A7%E5%85%A8
【转载】目标蛋白粗提方法
http://www.genecool.com/bbs/viewthread.php?tid=5508&highlight=%E7%9B%AE%E6%A0%87%E8%9B%8B%E7%99%BD%E7%B2%97%E6%8F%90%E6%96%B9%E6%B3%95
【转载】蛋白质提取的方法总汇
http://www.genecool.com/bbs/viewthread.php?tid=5204&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E6%8F%90%E5%8F%96%E7%9A%84%E6%96%B9%E6%B3%95%E6%80%BB%E6%B1%87
【转载】实验技术理论－生物大分子的制备
http://www.genecool.com/bbs/viewthread.php?tid=5382&highlight=%E5%AE%9E%E9%AA%8C%E6%8A%80%E6%9C%AF%E7%90%86%E8%AE%BA%EF%BC%8D%E7%94%9F%E7%89%A9%E5%A4%A7%E5%88%86%E5%AD%90%E7%9A%84%E5%88%B6%E5%A4%87
组织细胞破碎方法
对不起，没有找到匹配结果.
【求助】急求人类贴壁细胞总蛋白提取裂解液的配方
http://www.genecool.com/bbs/viewthread.php?tid=5775&highlight=%E6%80%A5%E6%B1%82%E4%BA%BA%E7%B1%BB%E8%B4%B4%E5%A3%81%E7%BB%86%E8%83%9E%E6%80%BB%E8%9B%8B%E7%99%BD%E6%8F%90%E5%8F%96%E8%A3%82%E8%A7%A3%E6%B6%B2%E7%9A%84%E9%85%8D%E6%96%B9

3.
蛋白质分离纯化
3.1
概论
蛋白质色谱基本概念
对不起，没有找到匹配结果。
【分享】生化分离技术－－柱层析分离
http://www.genecool.com/bbs/viewthread.php?tid=4146&highlight=%E7%94%9F%E5%8C%96%E5%88%86%E7%A6%BB%E6%8A%80%E6%9C%AF%EF%BC%8D%EF%BC%8D%E6%9F%B1%E5%B1%82%E6%9E%90%E5%88%86%E7%A6%BB
【转载】蛋白质分离纯化的一般程序
http://www.genecool.com/bbs/viewthread.php?tid=2186&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E5%88%86%E7%A6%BB%E7%BA%AF%E5%8C%96%E7%9A%84%E4%B8%80%E8%88%AC%E7%A8%8B%E5%BA%8F
【分享】蛋白质纯化的一般原则及方法选择
http://www.genecool.com/bbs/viewthread.php?tid=4419&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%BA%AF%E5%8C%96%E7%9A%84%E4%B8%80%E8%88%AC%E5%8E%9F%E5%88%99%E5%8F%8A%E6%96%B9%E6%B3%95%E9%80%89%E6%8B%A9
【上传】蛋白质纯化的方法选择
http://www.genecool.com/bbs/viewthread.php?tid=3735&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%BA%AF%E5%8C%96%E7%9A%84%E6%96%B9%E6%B3%95%E9%80%89%E6%8B%A9
蛋白质特性与分离纯化技术的选择
http://www.genecool.com/bbs/viewthread.php?tid=61&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%89%B9%E6%80%A7%E4%B8%8E%E5%88%86%E7%A6%BB%E7%BA%AF%E5%8C%96%E6%8A%80%E6%9C%AF%E7%9A%84%E9%80%89%E6%8B%A9
http://www.genecool.com/bbs/viewthread.php?tid=333&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%89%B9%E6%80%A7%E4%B8%8E%E5%88%86%E7%A6%BB%E7%BA%AF%E5%8C%96%E6%8A%80%E6%9C%AF%E7%9A%84%E9%80%89%E6%8B%A9
【原创】蛋白质纯化PPT
http://www.genecool.com/bbs/viewthread.php?tid=5400&highlight=%E3%80%90%E5%8E%9F%E5%88%9B%E3%80%91%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%BA%AF%E5%8C%96
【分享】蛋白质的分离与纯化
http://www.genecool.com/bbs/viewthread.php?tid=5257&highlight=%E3%80%90%E5%88%86%E4%BA%AB%E3%80%91%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%9A%84%E5%88%86%E7%A6%BB%E4%B8%8E%E7%BA%AF%E5%8C%96
【分享】液相色谱技术PPT
http://www.genecool.com/bbs/viewthread.php?tid=4194&highlight=%E6%B6%B2%E7%9B%B8%E8%89%B2%E8%B0%B1%E6%8A%80%E6%9C%AF
【分享】基因表达与蛋白质纯化技术探讨ppt（复旦大学）
http://www.genecool.com/bbs/viewthread.php?tid=3706&highlight=%E5%9F%BA%E5%9B%A0%E8%A1%A8%E8%BE%BE%E4%B8%8E%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%BA%AF%E5%8C%96%E6%8A%80%E6%9C%AF%E6%8E%A2%E8%AE%A8
【转载】蛋白质、多肽提取分离
http://bbs.genecool.com/viewthread.php?tid=2936&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E3%80%81%E5%A4%9A%E8%82%BD%E6%8F%90%E5%8F%96%E5%88%86%E7%A6%BB
酶的分离纯化方法简介
http://bbs.genecool.com/viewthread.php?tid=2751&highlight=%E9%85%B6%E7%9A%84%E5%88%86%E7%A6%BB%E7%BA%AF%E5%8C%96%E6%96%B9%E6%B3%95%E7%AE%80%E4%BB%8B
【转载】下游纯化工艺简介
http://bbs.genecool.com/viewthread.php?tid=2375&highlight=%E4%B8%8B%E6%B8%B8%E7%BA%AF%E5%8C%96%E5%B7%A5%E8%89%BA%E7%AE%80%E4%BB%8B
一些蛋白纯化有关的材料
http://bbs.genecool.com/viewthread.php?tid=2374&highlight=%E4%B8%80%E4%BA%9B%E8%9B%8B%E7%99%BD%E7%BA%AF%E5%8C%96%E6%9C%89%E5%85%B3%E7%9A%84%E6%9D%90%E6%96%99
【分享】蛋白质纯化－人人都会用到的实用技术
http://bbs.genecool.com/viewthread.php?tid=2121&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%BA%AF%E5%8C%96%EF%BC%8D%E4%BA%BA%E4%BA%BA%E9%83%BD%E4%BC%9A%E7%94%A8%E5%88%B0%E7%9A%84%E5%AE%9E%E7%94%A8%E6%8A%80%E6%9C%AF
蛋白质分离纯化的新技术
http://bbs.genecool.com/viewthread.php?tid=693&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E5%88%86%E7%A6%BB%E7%BA%AF%E5%8C%96%E7%9A%84%E6%96%B0%E6%8A%80%E6%9C%AF
蛋白柱常见问题和日常维护
http://bbs.genecool.com/viewthread.php?tid=1397&highlight=%E8%9B%8B%E7%99%BD%E6%9F%B1%E5%B8%B8%E8%A7%81%E9%97%AE%E9%A2%98%E5%92%8C%E6%97%A5%E5%B8%B8%E7%BB%B4%E6%8A%A4
层析法常用数据表及性质
http://bbs.genecool.com/viewthread.php?tid=1370&highlight=%E5%B1%82%E6%9E%90%E6%B3%95%E5%B8%B8%E7%94%A8%E6%95%B0%E6%8D%AE%E8%A1%A8%E5%8F%8A%E6%80%A7%E8%B4%A8
【转载】蛋白分离的缓冲液系统比较
http://bbs.genecool.com/viewthread.php?tid=2213&highlight=%E3%80%90%E8%BD%AC%E8%BD%BD%E3%80%91%E8%9B%8B%E7%99%BD%E5%88%86%E7%A6%BB%E7%9A%84%E7%BC%93%E5%86%B2%E6%B6%B2%E7%B3%BB%E7%BB%9F%E6%AF%94%E8%BE%83
http://bbs.genecool.com/viewthread.php?tid=5582&highlight=%E3%80%90%E8%BD%AC%E8%BD%BD%E3%80%91%E8%9B%8B%E7%99%BD%E5%88%86%E7%A6%BB%E7%9A%84%E7%BC%93%E5%86%B2%E6%B6%B2%E7%B3%BB%E7%BB%9F%E6%AF%94%E8%BE%83
蛋白纯化实验经验总结
http://bbs.genecool.com/viewthread.php?tid=773&highlight=%E8%9B%8B%E7%99%BD%E7%BA%AF%E5%8C%96%E5%AE%9E%E9%AA%8C%E7%BB%8F%E9%AA%8C%E6%80%BB%E7%BB%93
色谱常见问题问答
http://bbs.genecool.com/viewthread.php?tid=1777&highlight=%E8%89%B2%E8%B0%B1%E5%B8%B8%E8%A7%81%E9%97%AE%E9%A2%98%E9%97%AE%E7%AD%94
关于柱层析的实验方法和技巧（转贴）
http://bbs.genecool.com/viewthread.php?tid=1782&highlight=%E5%85%B3%E4%BA%8E%E6%9F%B1%E5%B1%82%E6%9E%90%E7%9A%84%E5%AE%9E%E9%AA%8C%E6%96%B9%E6%B3%95%E5%92%8C%E6%8A%80%E5%B7%A7
【转载】过柱的经验之谈
http://bbs.genecool.com/viewthread.php?tid=3124&highlight=%E8%BF%87%E6%9F%B1%E7%9A%84%E7%BB%8F%E9%AA%8C%E4%B9%8B%E8%B0%88
转贴：239个关于蛋白纯化的求助问题
http://bbs.genecool.com/viewthread.php?tid=16&highlight=239%E4%B8%AA%E5%85%B3%E4%BA%8E%E8%9B%8B%E7%99%BD%E7%BA%AF%E5%8C%96%E7%9A%84%E6%B1%82%E5%8A%A9%E9%97%AE%E9%A2%98
求助：关于凝胶柱的清洗问题
http://bbs.genecool.com/viewthread.php?tid=1367&highlight=%E5%85%B3%E4%BA%8E%E5%87%9D%E8%83%B6%E6%9F%B1%E7%9A%84%E6%B8%85%E6%B4%97%E9%97%AE%E9%A2%98
(求助)问题：蛋白质纯化，装柱，过柱应注意事项有哪些？
http://bbs.genecool.com/viewthread.php?tid=810&highlight=%E9%97%AE%E9%A2%98%EF%BC%9A%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%BA%AF%E5%8C%96%EF%BC%8C%E8%A3%85%E6%9F%B1%EF%BC%8C%E8%BF%87%E6%9F%B1%E5%BA%94%E6%B3%A8%E6%84%8F%E4%BA%8B%E9%A1%B9%E6%9C%89%E5%93%AA%E4%BA%9B
层析关于装柱和使用，有哪些需要注意的？
http://bbs.genecool.com/viewthread.php?tid=814&highlight=%E5%B1%82%E6%9E%90%E5%85%B3%E4%BA%8E%E8%A3%85%E6%9F%B1%E5%92%8C%E4%BD%BF%E7%94%A8%EF%BC%8C%E6%9C%89%E5%93%AA%E4%BA%9B%E9%9C%80%E8%A6%81%E6%B3%A8%E6%84%8F%E7%9A%84
【分享】蛋白纯化手册
http://bbs.genecool.com/viewthread.php?tid=7053&highlight=%E8%9B%8B%E7%99%BD%E7%BA%AF%E5%8C%96%E6%89%8B%E5%86%8C
【分享】关于过柱的实验方法和技巧
http://bbs.genecool.com/viewthread.php?tid=6868&highlight=%E5%85%B3%E4%BA%8E%E8%BF%87%E6%9F%B1%E7%9A%84%E5%AE%9E%E9%AA%8C%E6%96%B9%E6%B3%95%E5%92%8C%E6%8A%80%E5%B7%A7
【求助】层析柱应该怎么清洗
http://bbs.genecool.com/viewthread.php?tid=7191&highlight=%E5%B1%82%E6%9E%90%E6%9F%B1%E5%BA%94%E8%AF%A5%E6%80%8E%E4%B9%88%E6%B8%85%E6%B4%97

3.2
凝胶过滤层析
【转载】凝胶过滤层析法及其应用
http://bbs.genecool.com/viewthread.php?tid=4204&highlight=%E5%87%9D%E8%83%B6%E8%BF%87%E6%BB%A4%E5%B1%82%E6%9E%90%E6%B3%95%E5%8F%8A%E5%85%B6%E5%BA%94%E7%94%A8
凝胶层析技术
http://bbs.genecool.com/viewthread.php?tid=516&highlight=%E5%87%9D%E8%83%B6%E5%B1%82%E6%9E%90%E6%8A%80%E6%9C%AF
【分享】几种分子筛凝胶及应用
http://bbs.genecool.com/viewthread.php?tid=5187&highlight=%E5%87%A0%E7%A7%8D%E5%88%86%E5%AD%90%E7%AD%9B%E5%87%9D%E8%83%B6%E5%8F%8A%E5%BA%94%E7%94%A8
【分享】葡聚糖凝胶柱使用及注意事项
http://bbs.genecool.com/viewthread.php?tid=2759&highlight=%E8%91%A1%E8%81%9A%E7%B3%96%E5%87%9D%E8%83%B6%E6%9F%B1%E4%BD%BF%E7%94%A8%E5%8F%8A%E6%B3%A8%E6%84%8F%E4%BA%8B%E9%A1%B9
【分享】选择纯化凝胶的具体方法
http://bbs.genecool.com/viewthread.php?tid=1520&highlight=%E9%80%89%E6%8B%A9%E7%BA%AF%E5%8C%96%E5%87%9D%E8%83%B6%E7%9A%84%E5%85%B7%E4%BD%93%E6%96%B9%E6%B3%95
【求助】请问蛋白质过分子筛时为何分子量中等大小的蛋白先洗脱，分子量大的蛋白和分子量小的一起洗脱?
http://bbs.genecool.com/viewthread.php?tid=5539&highlight=%E8%AF%B7%E9%97%AE%E8%9B%8B%E7%99%BD%E8%B4%A8%E8%BF%87%E5%88%86%E5%AD%90%E7%AD%9B%E6%97%B6%E4%B8%BA%E4%BD%95%E5%88%86%E5%AD%90%E9%87%8F%E4%B8%AD%E7%AD%89%E5%A4%A7%E5%B0%8F%E7%9A%84%E8%9B%8B%E7%99%BD%E5%85%88%E6%B4%97%E8%84%B1%EF%BC%8C%E5%88%86%E5%AD%90%E9%87%8F%E5%A4%A7%E7%9A%84%E8%9B%8B%E7%99%BD%E5%92%8C%E5%88%86%E5%AD%90%E9%87%8F%E5%B0%8F%E7%9A%84%E4%B8%80
【求助】关于分子筛纯化的详细步骤
http://bbs.genecool.com/viewthread.php?tid=7274&highlight=%E5%85%B3%E4%BA%8E%E5%88%86%E5%AD%90%E7%AD%9B%E7%BA%AF%E5%8C%96%E7%9A%84%E8%AF%A6%E7%BB%86%E6%AD%A5%E9%AA%A4
【分享】凝胶过滤填料的分离范围表
http://bbs.genecool.com/viewthread.php?tid=6682&highlight=%E5%87%9D%E8%83%B6%E8%BF%87%E6%BB%A4%E5%A1%AB%E6%96%99%E7%9A%84%E5%88%86%E7%A6%BB%E8%8C%83%E5%9B%B4%E8%A1%A8

3.3
离子交换层析
离子色谱基本原理
http://bbs.genecool.com/viewthread.php?tid=644&highlight=%E7%A6%BB%E5%AD%90%E8%89%B2%E8%B0%B1%E5%9F%BA%E6%9C%AC%E5%8E%9F%E7%90%86
(分享)离子交换层析
http://bbs.genecool.com/viewthread.php?tid=3804&highlight=%28%E5%88%86%E4%BA%AB%29%E7%A6%BB%E5%AD%90%E4%BA%A4%E6%8D%A2%E5%B1%82%E6%9E%90
【转载】离心技术原理与类型
http://bbs.genecool.com/viewthread.php?tid=1522&highlight=%E7%A6%BB%E5%BF%83%E6%8A%80%E6%9C%AF%E5%8E%9F%E7%90%86%E4%B8%8E%E7%B1%BB%E5%9E%8B
关于离子交换柱纯化蛋白的问题?
http://bbs.genecool.com/viewthread.php?tid=154&highlight=%E5%85%B3%E4%BA%8E%E7%A6%BB%E5%AD%90%E4%BA%A4%E6%8D%A2%E6%9F%B1%E7%BA%AF%E5%8C%96%E8%9B%8B%E7%99%BD%E7%9A%84%E9%97%AE%E9%A2%98
【求助】请问离子交换的缓冲液中可以含甘油吗？
http://bbs.genecool.com/viewthread.php?tid=5538&highlight=%E8%AF%B7%E9%97%AE%E7%A6%BB%E5%AD%90%E4%BA%A4%E6%8D%A2%E7%9A%84%E7%BC%93%E5%86%B2%E6%B6%B2%E4%B8%AD%E5%8F%AF%E4%BB%A5%E5%90%AB%E7%94%98%E6%B2%B9%E5%90%97
【求助】请问关于DEAE－纤维素阴离子交换层析的问题？
http://bbs.genecool.com/viewthread.php?tid=6740&highlight=%E8%AF%B7%E9%97%AE%E5%85%B3%E4%BA%8EDEAE%EF%BC%8D%E7%BA%A4%E7%BB%B4%E7%B4%A0%E9%98%B4%E7%A6%BB%E5%AD%90%E4%BA%A4%E6%8D%A2%E5%B1%82%E6%9E%90%E7%9A%84%E9%97%AE%E9%A2%98
【分享】离子色谱分离方式和检测方式的选择
http://bbs.genecool.com/viewthread.php?tid=6390&highlight=%E7%A6%BB%E5%AD%90%E8%89%B2%E8%B0%B1%E5%88%86%E7%A6%BB%E6%96%B9%E5%BC%8F%E5%92%8C%E6%A3%80%E6%B5%8B%E6%96%B9%E5%BC%8F%E7%9A%84%E9%80%89%E6%8B%A9
【分享】离子交换层析纯化超氧化物歧化酶
http://bbs.genecool.com/viewthread.php?tid=6388&highlight=%E7%A6%BB%E5%AD%90%E4%BA%A4%E6%8D%A2%E5%B1%82%E6%9E%90%E7%BA%AF%E5%8C%96%E8%B6%85%E6%B0%A7%E5%8C%96%E7%89%A9%E6%AD%A7%E5%8C%96%E9%85%B6

3.4
亲和层析
亲和层析技术研究进展
http://bbs.genecool.com/viewthread.php?tid=586&highlight=%E4%BA%B2%E5%92%8C%E5%B1%82%E6%9E%90%E6%8A%80%E6%9C%AF%E7%A0%94%E7%A9%B6%E8%BF%9B%E5%B1%95
【原创】蛋白质相互作用实验方法
http://bbs.genecool.com/viewthread.php?tid=5792&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%9B%B8%E4%BA%92%E4%BD%9C%E7%94%A8%E5%AE%9E%E9%AA%8C%E6%96%B9%E6%B3%95
【求助】我想问一下抗体的纯化技术！
http://bbs.genecool.com/viewthread.php?tid=5421&highlight=%E6%88%91%E6%83%B3%E9%97%AE%E4%B8%80%E4%B8%8B%E6%8A%97%E4%BD%93%E7%9A%84%E7%BA%AF%E5%8C%96%E6%8A%80%E6%9C%AF
【求助】关于亲和层析回收率
http://bbs.genecool.com/viewthread.php?tid=1233&highlight=%E5%85%B3%E4%BA%8E%E4%BA%B2%E5%92%8C%E5%B1%82%E6%9E%90%E5%9B%9E%E6%94%B6%E7%8E%87
【求助】请问关于兔多抗的纯化问题
http://bbs.genecool.com/viewthread.php?tid=6742&highlight=%E8%AF%B7%E9%97%AE%E5%85%B3%E4%BA%8E%E5%85%94%E5%A4%9A%E6%8A%97%E7%9A%84%E7%BA%AF%E5%8C%96%E9%97%AE%E9%A2%98

3.5
聚焦色谱
【求助】层析聚焦的原理
http://bbs.genecool.com/viewthread.php?tid=4886&highlight=%E5%B1%82%E6%9E%90%E8%81%9A%E7%84%A6%E7%9A%84%E5%8E%9F%E7%90%86
蛋白质等电聚焦技术！
http://bbs.genecool.com/viewthread.php?tid=237&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%AD%89%E7%94%B5%E8%81%9A%E7%84%A6%E6%8A%80%E6%9C%AF

3.6
其它
【原创】蛋白质相互作用实验方法
http://bbs.genecool.com/viewthread.php?tid=5792&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%9B%B8%E4%BA%92%E4%BD%9C%E7%94%A8%E5%AE%9E%E9%AA%8C%E6%96%B9%E6%B3%95
【求助】关于蛋白质相互作用的实验方法
http://bbs.genecool.com/viewthread.php?tid=6192&highlight=%E5%85%B3%E4%BA%8E%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%9B%B8%E4%BA%92%E4%BD%9C%E7%94%A8%E7%9A%84%E5%AE%9E%E9%AA%8C%E6%96%B9%E6%B3%95
【求助】镍柱再生有什么好方法？
http://bbs.genecool.com/viewthread.php?tid=6519&highlight=%E9%95%8D%E6%9F%B1%E5%86%8D%E7%94%9F%E6%9C%89%E4%BB%80%E4%B9%88%E5%A5%BD%E6%96%B9%E6%B3%95
【求助】纳米粒子金纯化蛋白
http://bbs.genecool.com/viewthread.php?tid=7114&highlight=%E7%BA%B3%E7%B1%B3%E7%B2%92%E5%AD%90%E9%87%91%E7%BA%AF%E5%8C%96%E8%9B%8B%E7%99%BD
【分享】氨基酸的纸层析
http://bbs.genecool.com/viewthread.php?tid=6389&highlight=%E6%B0%A8%E5%9F%BA%E9%85%B8%E7%9A%84%E7%BA%B8%E5%B1%82%E6%9E%90

已效验有效，参照http://www.genecool.com/bbs/thread-11020-1-1.html，hamye已经给予部分奖励，如能将剩下部分整理完，可给予5-10点威望奖励，楼主更新部分我将复制到原整理贴，不影响奖励结果

请各位酷友原谅，非我不愿意整理，而是希望有需要的酷友在整理的过程中能看到自己想看的资料、讨论等，也希望有酷友因为帮助整理旧帖能得到换取基因库中资源的威望和酷币

nano
说的对呀
其实我主要是想找这方面的东西补课
因为我最近要做酶的分离提纯
没这方面的经验
其实在找这些资料的同时
也发现有些资料重复
全面整理还需要多人参与
如果可能
我将继续整理部分

继续
4.
蛋白质电泳
常用电泳技术简介
http://www.genecool.com/bbs/viewthread.php?tid=518&highlight=%E5%B8%B8%E7%94%A8%E7%94%B5%E6%B3%B3%E6%8A%80%E6%9C%AF%E7%AE%80%E4%BB%8B
【上传】电泳技术课件
http://www.genecool.com/bbs/viewthread.php?tid=5194&highlight=%E7%94%B5%E6%B3%B3%E6%8A%80%E6%9C%AF%E8%AF%BE%E4%BB%B6
聚丙烯酰胺凝胶电泳
http://www.genecool.com/bbs/viewthread.php?tid=680&highlight=%E8%81%9A%E4%B8%99%E7%83%AF%E9%85%B0%E8%83%BA%E5%87%9D%E8%83%B6%E7%94%B5%E6%B3%B3
二维聚丙烯酰胺凝胶电泳原理介绍
http://www.genecool.com/bbs/viewthread.php?tid=1391&highlight=%E8%81%9A%E4%B8%99%E7%83%AF%E9%85%B0%E8%83%BA%E5%87%9D%E8%83%B6%E7%94%B5%E6%B3%B3
SDS-聚丙烯酰胺凝胶电泳和Western转印的常见问题
http://www.genecool.com/bbs/viewthread.php?tid=1405&highlight=%E8%81%9A%E4%B8%99%E7%83%AF%E9%85%B0%E8%83%BA%E5%87%9D%E8%83%B6%E7%94%B5%E6%B3%B3
普通的聚丙烯酰胺凝胶电泳的操作
http://www.genecool.com/bbs/viewthread.php?tid=8709&highlight=%E8%81%9A%E4%B8%99%E7%83%AF%E9%85%B0%E8%83%BA%E5%87%9D%E8%83%B6%E7%94%B5%E6%B3%B3
丙稀酰胺凝胶配制经验大家谈：
http://www.genecool.com/bbs/viewthread.php?tid=5327&highlight=%E4%B8%99%E7%A8%80%E9%85%B0%E8%83%BA%E5%87%9D%E8%83%B6%E9%85%8D%E5%88%B6%E7%BB%8F%E9%AA%8C%E5%A4%A7%E5%AE%B6%E8%B0%88
蛋白质非变性电泳技术支持，有问题尽管问，大家一起讨论！
http://www.genecool.com/bbs/viewthread.php?tid=413&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E9%9D%9E%E5%8F%98%E6%80%A7%E7%94%B5%E6%B3%B3%E6%8A%80%E6%9C%AF%E6%94%AF%E6%8C%81%EF%BC%8C%E6%9C%89%E9%97%AE%E9%A2%98%E5%B0%BD%E7%AE%A1%E9%97%AE%EF%BC%8C%E5%A4%A7%E5%AE%B6%E4%B8%80%E8%B5%B7%E8%AE%A8%E8%AE%BA
SDS－PAGE电泳过程中常见问题以及解决方法：
http://www.genecool.com/bbs/viewthread.php?tid=54&highlight=SDS%EF%BC%8DPAGE%E7%94%B5%E6%B3%B3%E8%BF%87%E7%A8%8B%E4%B8%AD%E5%B8%B8%E8%A7%81%E9%97%AE%E9%A2%98%E4%BB%A5%E5%8F%8A%E8%A7%A3%E5%86%B3%E6%96%B9%E6%B3%95
SDS-PAGE及Western Blotting中遇到的问题
http://www.genecool.com/bbs/viewthread.php?tid=2286&highlight=SDS-PAGE%E5%8F%8AWestern%2BBlotting%E4%B8%AD%E9%81%87%E5%88%B0%E7%9A%84%E9%97%AE%E9%A2%98
SDS-PAGE电泳小问答
http://www.genecool.com/bbs/viewthread.php?tid=698&highlight=SDS-PAGE%E7%94%B5%E6%B3%B3%E5%B0%8F%E9%97%AE%E7%AD%94


求助：怎样根据蛋白质分子量大小配置分离胶
对不起，没有找到匹配结果。
【分享】电泳技术
http://www.genecool.com/bbs/viewthread.php?tid=5345&highlight=%E3%80%90%E5%88%86%E4%BA%AB%E3%80%91%E7%94%B5%E6%B3%B3%E6%8A%80%E6%9C%AF
【求助】跑SDS-PAGE电泳的无奈!
http://www.genecool.com/bbs/viewthread.php?tid=4909&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91%E8%B7%91SDS-PAGE%E7%94%B5%E6%B3%B3%E7%9A%84%E6%97%A0%E5%A5%88
【求助】丙稀酰胺毒性问题
http://www.genecool.com/bbs/viewthread.php?tid=5193&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91%E4%B8%99%E7%A8%80%E9%85%B0%E8%83%BA%E6%AF%92%E6%80%A7%E9%97%AE%E9%A2%98
（求助）在SDS-PAGE时，缓冲液中的甘氨酸成分的好坏对样品的条带会产生什么影响？
http://www.genecool.com/bbs/viewthread.php?tid=3300&highlight=%EF%BC%88%E6%B1%82%E5%8A%A9%EF%BC%89%E5%9C%A8SDS-PAGE%E6%97%B6%EF%BC%8C%E7%BC%93%E5%86%B2%E6%B6%B2%E4%B8%AD%E7%9A%84%E7%94%98%E6%B0%A8%E9%85%B8%E6%88%90%E5%88%86%E7%9A%84%E5%A5%BD%E5%9D%8F%E5%AF%B9%E6%A0%B7%E5%93%81%E7%9A%84%E6%9D%A1%E5%B8%A6%E4%BC%9A%E4%BA%A7%E7%94%9F%E4%BB%80%E4%B9%88%E5%BD%B1
【求助】SDS-PAGE电泳上样缓冲液变红是什么原因？
http://www.genecool.com/bbs/viewthread.php?tid=3771&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91SDS-PAGE%E7%94%B5%E6%B3%B3%E4%B8%8A%E6%A0%B7%E7%BC%93%E5%86%B2%E6%B6%B2%E5%8F%98%E7%BA%A2%E6%98%AF%E4%BB%80%E4%B9%88%E5%8E%9F%E5%9B%A0%EF%BC%9F
【求助】SDS胶里面加甘油是什么原理？？？
http://www.genecool.com/bbs/viewthread.php?tid=1760&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91SDS%E8%83%B6%E9%87%8C%E9%9D%A2%E5%8A%A0%E7%94%98%E6%B2%B9%E6%98%AF%E4%BB%80%E4%B9%88%E5%8E%9F%E7%90%86

【求助】不溶性蛋白的SDS-PAGE
http://www.genecool.com/bbs/viewthread.php?tid=1017&highlight=%5B%E6%B1%82%E5%8A%A9%5D%E4%B8%8D%E6%BA%B6%E6%80%A7%E8%9B%8B%E7%99%BD%E7%9A%84
【求助】蛋白SDS-PAGE电泳中常用的loading buffer中的那些成分都有什么作用啊？http://www.genecool.com/bbs/viewthread.php?tid=1531&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91%E8%9B%8B%E7%99%BDSDS-PAGE%E7%94%B5%E6%B3%B3%E4%B8%AD%E5%B8%B8%E7%94%A8%E7%9A%84loading%2Bbuffer%E4%B8%AD%E7%9A%84%E9%82%A3%E4%BA%9B%E6%88%90%E5%88%86
聚丙烯酰胺电泳分离蛋白质边缘2条带效果很差，如何解决？
http://www.genecool.com/bbs/viewthread.php?tid=844&highlight=%E8%81%9A%E4%B8%99%E7%83%AF%E9%85%B0%E8%83%BA%E7%94%B5%E6%B3%B3%E5%88%86%E7%A6%BB%E8%9B%8B%E7%99%BD%E8%B4%A8%E8%BE%B9%E7%BC%982%E6%9D%A1%E5%B8%A6%E6%95%88%E6%9E%9C%E5%BE%88%E5%B7%AE%EF%BC%8C%E5%A6%82%E4%BD%95%E8%A7%A3%E5%86%B3
（求助）蛋白电泳问题
http://www.genecool.com/bbs/viewthread.php?tid=1776&highlight=%EF%BC%88%E6%B1%82%E5%8A%A9%EF%BC%89%E8%9B%8B%E7%99%BD%E7%94%B5%E6%B3%B3%E9%97%AE%E9%A2%98
【求助】关于蛋白质活性电泳
http://www.genecool.com/bbs/viewthread.php?tid=4593&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91%E5%85%B3%E4%BA%8E%E8%9B%8B%E7%99%BD%E8%B4%A8%E6%B4%BB%E6%80%A7%E7%94%B5%E6%B3%B3
求助:请问在配制聚炳酰胺时APS和TEMED的比例对蛋白条带有影响吗?
http://www.genecool.com/bbs/viewthread.php?tid=1355&highlight=%E6%B1%82%E5%8A%A9%3A%E8%AF%B7%E9%97%AE%E5%9C%A8%E9%85%8D%E5%88%B6%E8%81%9A%E7%82%B3%E9%85%B0%E8%83%BA%E6%97%B6APS%E5%92%8CTEMED%E7%9A%84%E6%AF%94%E4%BE%8B%E5%AF%B9%E8%9B%8B%E7%99%BD%E6%9D%A1%E5%B8%A6%E6%9C%89%E5%BD%B1%E5%93%8D%E5%90%97
（求助）蛋白在SDS凝胶中异常迁移的原因及对策？
http://www.genecool.com/bbs/viewthread.php?tid=371&highlight=%EF%BC%88%E6%B1%82%E5%8A%A9%EF%BC%89%E8%9B%8B%E7%99%BD%E5%9C%A8SDS%E5%87%9D%E8%83%B6%E4%B8%AD%E5%BC%82%E5%B8%B8%E8%BF%81%E7%A7%BB%E7%9A%84%E5%8E%9F%E5%9B%A0%E5%8F%8A%E5%AF%B9%E7%AD%96
大家来探讨一下：做PAGE胶的时候有时候梳子特别难拔，是什么原因？
http://www.genecool.com/bbs/viewthread.php?tid=485&highlight=%E5%A4%A7%E5%AE%B6%E6%9D%A5%E6%8E%A2%E8%AE%A8%E4%B8%80%E4%B8%8B%EF%BC%9A%E5%81%9APAGE%E8%83%B6%E7%9A%84%E6%97%B6%E5%80%99%E6%9C%89%E6%97%B6%E5%80%99%E6%A2%B3%E5%AD%90%E7%89%B9%E5%88%AB%E9%9A%BE%E6%8B%94%EF%BC%8C%E6%98%AF%E4%BB%80%E4%B9%88%E5%8E%9F%E5%9B%A0

【求助】PAGE高手请进!!小弟有话要说
http://www.genecool.com/bbs/viewthread.php?tid=7211&highlight=%E6%B1%82%E5%8A%A9%E3%80%91PAGE%E9%AB%98%E6%89%8B%E8%AF%B7%E8%BF%9B%21%21%E5%B0%8F%E5%BC%9F%E6%9C%89%E8%AF%9D%E8%A6%81%E8%AF%B4
【求助】大家帮忙看一看电泳图
http://www.genecool.com/bbs/viewthread.php?tid=6650&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91%E5%A4%A7%E5%AE%B6%E5%B8%AE%E5%BF%99%E7%9C%8B%E4%B8%80%E7%9C%8B%E7%94%B5%E6%B3%B3%E5%9B%BE
【讨论】蛋白纯化出来放置一段时间后跑胶会在胶的上面多出了几条很小的带，是什么原因呢？
http://www.genecool.com/bbs/viewthread.php?tid=6463&highlight=%E3%80%90%E8%AE%A8%E8%AE%BA%E3%80%91%E8%9B%8B%E7%99%BD%E7%BA%AF%E5%8C%96%E5%87%BA%E6%9D%A5%E6%94%BE%E7%BD%AE%E4%B8%80%E6%AE%B5%E6%97%B6%E9%97%B4%E5%90%8E%E8%B7%91%E8%83%B6%E4%BC%9A%E5%9C%A8%E8%83%B6%E7%9A%84%E4%B8%8A%E9%9D%A2%E5%A4%9A%E5%87%BA%E4%BA%86%E5%87%A0%E6%9D%A1%E5%BE%88%E5%B0%8F%E7%9A%84%E5%B8%A6%EF%BC%8C%E6%98%AF%E4%BB%80%E4%B9%88%E5%8E%9F%E5%9B%A0
【求助】SDS-PAGE电泳
http://www.genecool.com/bbs/viewthread.php?tid=6725&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91SDS-PAGE%E7%94%B5%E6%B3%B3
【求助】SDS-PAGE电泳结果分析
http://www.genecool.com/bbs/viewthread.php?tid=7846&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91SDS-PAGE%E7%94%B5%E6%B3%B3
【求助】怎样用电泳的方法分离小分子活性多肽?
http://www.genecool.com/bbs/viewthread.php?tid=6530&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91%E6%80%8E%E6%A0%B7%E7%94%A8%E7%94%B5%E6%B3%B3%E7%9A%84%E6%96%B9%E6%B3%95%E5%88%86%E7%A6%BB%E5%B0%8F%E5%88%86%E5%AD%90%E6%B4%BB%E6%80%A7%E5%A4%9A%E8%82%BD
【分享】常用蛋白染色方法
http://www.genecool.com/bbs/viewthread.php?tid=5119&highlight=%E3%80%90%E5%88%86%E4%BA%AB%E3%80%91%E5%B8%B8%E7%94%A8%E8%9B%8B%E7%99%BD%E6%9F%93%E8%89%B2%E6%96%B9%E6%B3%95
【转载】blue sliver凝胶染色法
http://www.genecool.com/bbs/viewthread.php?tid=4492&highlight=%E3%80%90%E8%BD%AC%E8%BD%BD%E3%80%91blue%2Bsliver%E5%87%9D%E8%83%B6%E6%9F%93%E8%89%B2%E6%B3%95
【求助】蛋白质的KCL快速染色原理是什么？
http://www.genecool.com/bbs/viewthread.php?tid=4800&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91%E8%9B%8B%E7%99%BD%E8%B4%A8%E7%9A%84KCL%E5%BF%AB%E9%80%9F%E6%9F%93%E8%89%B2%E5%8E%9F%E7%90%86%E6%98%AF%E4%BB%80%E4%B9%88
蛋白质银染问题
http://www.genecool.com/bbs/viewthread.php?tid=899&highlight=%E8%9B%8B%E7%99%BD%E8%B4%A8%E9%93%B6%E6%9F%93%E9%97%AE%E9%A2%98
【分享】做银染时候用到的有毒试剂
http://www.genecool.com/bbs/viewthread.php?tid=7194&highlight=%E3%80%90%E5%88%86%E4%BA%AB%E3%80%91%E5%81%9A%E9%93%B6%E6%9F%93%E6%97%B6%E5%80%99%E7%94%A8%E5%88%B0%E7%9A%84%E6%9C%89%E6%AF%92%E8%AF%95%E5%89%82
考马斯亮蓝蛋白胶快速染色液常见问题
http://www.genecool.com/bbs/viewthread.php?tid=1404&highlight=%E8%80%83%E9%A9%AC%E6%96%AF%E4%BA%AE%E8%93%9D%E8%9B%8B%E7%99%BD%E8%83%B6%E5%BF%AB%E9%80%9F%E6%9F%93%E8%89%B2%E6%B6%B2%E5%B8%B8%E8%A7%81%E9%97%AE%E9%A2%98
请问：考马斯亮蓝与氯化铜对蛋白染色有何区别？
http://www.genecool.com/bbs/viewthread.php?tid=803&highlight=%E8%AF%B7%E9%97%AE%EF%BC%9A%E8%80%83%E9%A9%AC%E6%96%AF%E4%BA%AE%E8%93%9D%E4%B8%8E%E6%B0%AF%E5%8C%96%E9%93%9C%E5%AF%B9%E8%9B%8B%E7%99%BD%E6%9F%93%E8%89%B2%E6%9C%89%E4%BD%95%E5%8C%BA%E5%88%AB
【转载】双向电泳研究进展
http://www.genecool.com/bbs/viewthread.php?tid=4491&highlight=%E3%80%90%E8%BD%AC%E8%BD%BD%E3%80%91%E5%8F%8C%E5%90%91%E7%94%B5%E6%B3%B3%E7%A0%94%E7%A9%B6%E8%BF%9B%E5%B1%95
【转载】双向电泳
http://www.genecool.com/bbs/viewthread.php?tid=5123&highlight=%E3%80%90%E8%BD%AC%E8%BD%BD%E3%80%91%E5%8F%8C%E5%90%91%E7%94%B5%E6%B3%B3
【转载】双向电泳完整的操作步骤
http://www.genecool.com/bbs/viewthread.php?tid=1419&highlight=%E3%80%90%E8%BD%AC%E8%BD%BD%E3%80%91%E5%8F%8C%E5%90%91%E7%94%B5%E6%B3%B3
【分享】双向电泳
http://www.genecool.com/bbs/viewthread.php?tid=4231&highlight=%E3%80%90%E5%88%86%E4%BA%AB%E3%80%91%E5%8F%8C%E5%90%91%E7%94%B5%E6%B3%B3
【分享】双向电泳实验过程及相关溶液配置
http://www.genecool.com/bbs/viewthread.php?tid=5024&highlight=%E3%80%90%E5%88%86%E4%BA%AB%E3%80%91%E5%8F%8C%E5%90%91%E7%94%B5%E6%B3%B3
【分享】双向电泳资料
http://www.genecool.com/bbs/viewthread.php?tid=7939&highlight=%E3%80%90%E5%88%86%E4%BA%AB%E3%80%91%E5%8F%8C%E5%90%91%E7%94%B5%E6%B3%B3
【分享】双向电泳样品缓冲液选用的基本原则
http://www.genecool.com/bbs/viewthread.php?tid=5035&highlight=%E3%80%90%E5%88%86%E4%BA%AB%E3%80%91%E5%8F%8C%E5%90%91%E7%94%B5%E6%B3%B3
【上传】双向电泳操作手册
http://www.genecool.com/bbs/viewthread.php?tid=4195&highlight=%E3%80%90%E4%B8%8A%E4%BC%A0%E3%80%91%E5%8F%8C%E5%90%91%E7%94%B5%E6%B3%B3%E6%93%8D%E4%BD%9C%E6%89%8B%E5%86%8C
双向电泳完整操作步骤
http://www.genecool.com/bbs/viewthread.php?tid=3810&highlight=%E5%8F%8C%E5%90%91%E7%94%B5%E6%B3%B3%E5%AE%8C%E6%95%B4%E6%93%8D%E4%BD%9C%E6%AD%A5%E9%AA%A4
【转载】双向电泳完整的操作步骤
http://www.genecool.com/bbs/viewthread.php?tid=1419&highlight=%E3%80%90%E8%BD%AC%E8%BD%BD%E3%80%91%E5%8F%8C%E5%90%91%E7%94%B5%E6%B3%B3%E5%AE%8C%E6%95%B4%E7%9A%84%E6%93%8D%E4%BD%9C%E6%AD%A5%E9%AA%A4
【分享】双向电泳样品缓冲液选用的基本原则
http://www.genecool.com/bbs/viewthread.php?tid=5035&highlight=%E3%80%90%E5%88%86%E4%BA%AB%E3%80%91%E5%8F%8C%E5%90%91%E7%94%B5%E6%B3%B3%E6%A0%B7%E5%93%81%E7%BC%93%E5%86%B2%E6%B6%B2%E9%80%89%E7%94%A8%E7%9A%84%E5%9F%BA%E6%9C%AC%E5%8E%9F%E5%88%99
【转载】美国实验室wetern方法
http://www.genecool.com/bbs/viewthread.php?tid=4947&highlight=%E3%80%90%E8%BD%AC%E8%BD%BD%E3%80%91%E7%BE%8E%E5%9B%BD%E5%AE%9E%E9%AA%8C%E5%AE%A4wetern%E6%96%B9%E6%B3%95
【分享】我前几天去中科大听的讲座——westernblot
对不起，没有找到匹配结果。
求助：怎样用western blotting对蛋白质定量？
http://www.genecool.com/bbs/viewthread.php?tid=1456&highlight=%E6%B1%82%E5%8A%A9%EF%BC%9A%E6%80%8E%E6%A0%B7%E7%94%A8western%2Bblotting%E5%AF%B9%E8%9B%8B%E7%99%BD%E8%B4%A8%E5%AE%9A%E9%87%8F
请教：关于westernblot转膜
http://www.genecool.com/bbs/viewthread.php?tid=999&highlight=%E8%AF%B7%E6%95%99%EF%BC%9A%E5%85%B3%E4%BA%8Ewesternblot%E8%BD%AC%E8%86%9C
初做western blot出现的几个问题
http://www.genecool.com/bbs/viewthread.php?tid=609&highlight=%E5%88%9D%E5%81%9Awestern%2Bblot%E5%87%BA%E7%8E%B0%E7%9A%84%E5%87%A0%E4%B8%AA%E9%97%AE%E9%A2%98

western显影常见现象、原因及解决方案
http://www.genecool.com/bbs/viewthread.php?tid=455&highlight=western%E6%98%BE%E5%BD%B1%E5%B8%B8%E8%A7%81%E7%8E%B0%E8%B1%A1%E3%80%81%E5%8E%9F%E5%9B%A0%E5%8F%8A%E8%A7%A3%E5%86%B3%E6%96%B9%E6%A1%88

【分享】western blot 操作流程！
http://www.genecool.com/bbs/viewthread.php?tid=7109&highlight=%E3%80%90%E5%88%86%E4%BA%AB%E3%80%91western%2Bblot%2B%E6%93%8D%E4%BD%9C%E6%B5%81%E7%A8%8B
【求助】急！！请大家帮我分析分析Western blot 条带不好的原因
http://www.genecool.com/bbs/viewthread.php?tid=7277&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91%E6%80%A5%EF%BC%81%EF%BC%81%E8%AF%B7%E5%A4%A7%E5%AE%B6%E5%B8%AE%E6%88%91%E5%88%86%E6%9E%90%E5%88%86%E6%9E%90Western%2Bblot%2B%E6%9D%A1%E5%B8%A6%E4%B8%8D%E5%A5%BD%E7%9A%84%E5%8E%9F%E5%9B%A0
【求助】电泳完，胶能否保存几天再转膜
http://www.genecool.com/bbs/viewthread.php?tid=6477&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91%E7%94%B5%E6%B3%B3%E5%AE%8C%EF%BC%8C%E8%83%B6%E8%83%BD%E5%90%A6%E4%BF%9D%E5%AD%98%E5%87%A0%E5%A4%A9%E5%86%8D%E8%BD%AC%E8%86%9C
【求助】tris-tricine电泳疑惑
http://www.genecool.com/bbs/viewthread.php?tid=4608&highlight=%E3%80%90%E6%B1%82%E5%8A%A9%E3%80%91tris-tricine%E7%94%B5%E6%B3%B3%E7%96%91%E6%83%91
【转载】醋酸纤维素薄膜电泳的特点
http://www.genecool.com/bbs/viewthread.php?tid=3368&highlight=%E3%80%90%E8%BD%AC%E8%BD%BD%E3%80%91%E9%86%8B%E9%85%B8%E7%BA%A4%E7%BB%B4%E7%B4%A0%E8%96%84%E8%86%9C%E7%94%B5%E6%B3%B3%E7%9A%84%E7%89%B9%E7%82%B9
蛋白等电聚焦凝胶电泳技术原理：
http://www.genecool.com/bbs/viewthread.php?tid=59&highlight=%E8%9B%8B%E7%99%BD%E7%AD%89%E7%94%B5%E8%81%9A%E7%84%A6%E5%87%9D%E8%83%B6%E7%94%B5%E6%B3%B3%E6%8A%80%E6%9C%AF%E5%8E%9F%E7%90%86
有人做过梯度凝胶电泳吗？能介绍下吗？
http://www.genecool.com/bbs/viewthread.php?tid=1392&highlight=%E6%9C%89%E4%BA%BA%E5%81%9A%E8%BF%87%E6%A2%AF%E5%BA%A6%E5%87%9D%E8%83%B6%E7%94%B5%E6%B3%B3%E5%90%97%EF%BC%9F%E8%83%BD%E4%BB%8B%E7%BB%8D%E4%B8%8B%E5%90%97 【分享】圆盘电泳
http://www.genecool.com/bbs/viewthread.php?tid=6393&highlight=%E3%80%90%E5%88%86%E4%BA%AB%E3%80%91%E5%9C%86%E7%9B%98%E7%94%B5%E6%B3%B3