pCAMBIA w; k8 Y* |% _% J
| Plant Selection Gene , {. w: Y! R7 H
| Bacterial Selection Gene - F; q6 {: O2 d6 ?+ @' T
| Polylinker 4 y/ M6 f' r/ Y! e- A
| Reporter Gene 1 q2 o/ } ]# ]. L- `+ v& [; V# V" J
| T-DNA size (bp) 1 m7 t( f! h7 M' k7 F
| Reading Frame or lacZ
2 f$ |) M/ u! z j | Vector family % } b8 x1 }" w# ]! n8 ~, a" r4 s
| Map
* l: W k. \* Y9 L- ? |
2301 1 f0 _* Z6 @/ w/ W, e
| nptII
3 ~- X; B" P6 w1 |) Q | kan* \2 B; O* J9 m- ~# S) x
| pUC18
) Y% w9 N5 r* @( x | gusA ; k4 L9 A- B9 S% @% W) k* X$ K
| 5391
5 \$ u# @/ Z. [3 N: \: r | -% V" N @" s/ e% X( R
| GIS
* F1 W+ O& q* B& _" Y( K4 B) V | view map $ }6 t5 V: Q) y0 N% u/ x; i% z
|
2300
) \; i5 E& n7 R W | nptII
" e- l* x( ]# W; c% @* Q+ N( a | kan% j$ Y, Z2 q1 i1 h5 V6 H4 d
| pUC18
# j# j9 D u K5 ~% w5 [+ R | -
Q' m! n) d) i K7 y4 E | 2512# A2 C7 G- Q9 G- P! E" H% @; q9 [) T
| -# x6 P" j& X" ^1 j% L6 ?3 E2 z' b
| MSV) W3 Z) z! G# |) I
| view map
7 N' w: j/ c2 F9 l! a3 p |
2201
2 ^" }: `: g( @ | nptII , ]+ ?' V v# }% t% Y8 [; {
| cmr% }" d2 s7 o( B% w3 ~& t9 c5 \
| pUC18
8 N& o2 C/ M' m! D) I/ L0 A0 ^ | gusA
+ j! u3 z7 E3 M4 A' @ | 53913 i4 D. t" d! g3 o, Z' J2 d v
| -# ?' S6 v1 B- n4 U+ _
| GIS% @3 h, t4 I( R& k# w; R
| view map
% _% D6 \; t& g+ E7 F9 f |
2200 8 u* |$ _/ ~ h
| nptII
4 [, `: L: h: J: j p | cmr
# Y" \! A/ N* M: J: W | pUC18! X! ]7 H& [$ K
| -, a1 P; i/ G$ i
| 2512( w5 X. o; p% W: w, o- S
| -* L- L8 w# J8 q& H: R/ i3 N& H& {
| MSV" w) X: \- }1 O. }: r% ?( s
| view map & z% V: X. C' @# Z' e8 g6 F" I& G
|
1391
2 q3 {2 d, y1 s9 Z! _+ r# U | hptII ( {8 R' b: a8 n0 l: }
| kan) z2 X: Z9 |' o
| pUC94 z! m4 b: ?$ A% r, d7 D& G8 m& r
| gusA
" R! r, S! \# ^/ B | 4427* _; O1 z# e T4 L: z
| -
% ]& d& J; h- R7 L7 V) L | FUV
. O" S7 c# @& q8 p1 q K- G | view map
. }8 S* \9 V, o4 e8 G6 j0 J |
1391Z
1 L2 m6 K8 |3 Y" Z | hptII
+ F+ t% j6 C6 ]; G | kan
% I2 u. o1 o6 } | pUC9
N N6 J5 `+ C. c | gusA
) N7 K) I* ^( v/ }9 j! C/ K | 4984
( r! h9 f' n8 q% f4 B$ S | lacZ * U: A: ?) K# t. n$ x `) K
| PCV
7 @4 S* O* b0 c4 F/ Z* m6 T& l- w, I | view map ; \ F3 R) B# \5 ^! E- z
|
1391Xc ! G5 d# d( k# u; ]1 [2 S2 p1 A
| hptII 3 c/ y+ z: s0 K$ l; i$ v9 ]6 M
| kan( q/ L# [, s/ a, G& ?( P7 C
| pUC9- b% I( R+ R# |$ E
| gusA 3 [/ g3 {; ~7 o0 D: L" e
| -& n5 s, { T+ B( d* S
| c! j7 K$ y9 [' A' M N2 N$ ? Z
| FUV
! L, l6 ^( @2 o6 G& S" w9 B) Z | view map 8 R, ^* Z1 a( `* h7 q. t% y! Z
|
1391Xb 5 G2 r5 @+ {8 Q' g8 Q
| hptII ' `& `9 [% v/ _4 z
| kan
$ Y& H. i% Q+ ?4 I: x# ~( I+ l- |; O | pUC9' I; e2 j' g6 Q$ h& g2 c |) [. t
| gusA " x) m3 {1 o/ H# B) J' q) L$ a
| -6 ^# K3 K9 K* n/ p, H
| b
4 T1 q9 Y/ r: }5 A3 [6 c | FUV+ Q v% j; s& V% E2 S
| view map
) g# u% ?1 W+ h |
1391Xa
% w( Y* t9 \6 m* q; L | hptII ! y \3 ]9 p! R: Z6 | Y
| kan
. I4 t2 o4 y: r% L( U | pUC9
/ \- v3 {. |/ o, Z+ f1 J | gusA ) N1 y& _( p7 |) {
| -9 W( z5 L8 n* b* J
| a J3 \- S# G( c, r7 ?. C! j+ h
| FUV
9 M' x- ?9 x# k+ L s8 D | view map
/ L$ l6 G) }. b+ L |
1390
) N$ |8 V. m5 r {8 D | hptII
& ]" @* {) q" z& t | kan9 N" H4 \4 A" x4 m# S8 _ f
| pUC9
( t- K+ f" {2 U0 s" ^2 k5 u4 i. V | -
/ H7 V a" v \+ Q# B8 W9 a8 ^& P | 26309 x! T4 e: T" B
| -
/ \, O; E! C! o# @( B1 T! Q | MSV
) k9 ` Z9 _( |1 p! ]2 I; |. r9 q( u | view map " ]' V# }0 {8 G& g9 j
|
1381Z
& I3 U# v% ^1 O | hptII }& M k# |2 f
| kan0 v; J* c' C8 i3 [
| pUC8
+ y0 \1 i d/ R | gusA
' S8 q# n1 M* I+ s. T! S% [# A | 4984
8 r2 d8 w* H* c0 \( R | lacZ ) Z) U, D" f! s. r' J8 _) @8 ^
| PCV. a0 A; p8 o/ R
| view map
0 S# v ~$ U3 [ |
1381Xc
|7 N, y5 }) T* r2 ` | hptII
0 Z6 x$ n1 O( w: a+ C Y! N | kan
7 `$ ] Y0 Q& q7 I, o, _; z. ? | pUC8
% O( U: R0 D1 a" g" s | gusA 2 R$ Q! f' c6 K0 W2 P
| -
& n8 Q1 E. a8 c1 A/ T" F | c' A, h3 b9 F0 _- t
| FUV
/ h+ t" \2 M$ `- w | view map ' D# Y+ x* N# q' e
|
1381Xb 0 k% ~4 K0 g# \8 c% Z
| hptII
+ F# L9 G. q% D& t4 ]4 w: i | kan
7 s- c: A. l5 V4 p+ y9 B0 J: [ | pUC8
% {' n7 f! \ [# p | gusA 9 s- ?1 `) l1 _' }. j
| -; ^4 q& S6 T4 L0 E( G5 s3 N
| b
3 {5 J) _: F0 R1 g" b; } | FUV; b1 w U \' M. X6 ^/ _: ~& | X9 B
| 1 D% @; s* v" I3 n2 s
|
1381Xa . m+ ]7 z! f! @* n
| hptII
5 F" f9 v0 [( h0 i | kan
2 {9 e- d B1 o' O$ d3 Z& U- B$ k | pUC8, |0 e3 _$ m, U
| gusA # O# T# N, W Z
| -6 D4 R; N; l2 s7 M/ m
| a
( }( [1 P5 G" p | FUV
, Q) X1 D* d7 U! @) x | view map
2 p2 n2 c1 K" W, }$ q8 G |
1380 . [2 s1 ~8 e$ C2 i% U6 R
| hptII % F, }9 `/ I/ R
| kan
" i1 `7 o/ V1 r- m7 g# j | pUC8
7 }, @' k1 E5 a. E8 z3 g/ T+ F- g | -3 ^+ u% L: u) P& n/ m/ T
| 2630& o5 q- A6 |- x. V ]
| -: k( ?& I/ _! m" ^0 V
| MSV
$ ?- H6 e3 L( C- w* _8 D | view map
- d) S' H2 W8 h8 _ |
1304
" E; B! E8 j2 r* e9 ~# O' ? | hptII
6 y! T; g) K: g7 H0 q% h$ [ | kan& ]: j9 d9 f; e8 l. l! {
| pUC183 v( U& y8 H( `1 o' f$ ^$ s
| gfp:gusA
6 m x1 O6 l' I% D' J# L/ v: D | 6128
/ R7 q6 c' i# U+ J | -
k! }! b1 E9 N$ l; m" | | GFP, R/ M @1 Q( i; j8 R
| view map
3 `1 v. b+ ?) Y$ R |
1303 4 H& C4 R6 t& j( ^; @$ K
| hptII
# C$ H5 `2 d' X+ R) y, M! \ | kan
( Z, X3 O9 v \! w3 W2 l& Z | pUC181 E P1 ^9 ?1 \5 ]
| gusA:gfp
+ Z" b# ?/ Z1 {1 K2 p( ] | 6128
+ P2 r3 \4 o6 e i5 j; Y | -
( G1 l L4 X! j/ J) X | GFP
1 v) G# [6 D2 i3 i* ?# C | view map / R5 j0 D- I9 K6 }, c* W! N
|
1302 7 L2 s s( V z1 p& f. a# \
| hptII
9 B( r/ v/ B7 U | kan
+ ` ]" w5 W# f+ l3 M | pUC184 _, J+ y' |3 J0 N: l7 L
| gfp " d' e' v* _" h& X/ W6 Q; @3 c
| 4316$ z# b' }) j7 g$ }
| -' v/ D4 Y9 ]" ]4 G% B
| GFP( R1 r/ M+ {9 H8 o
| view map
2 T3 |+ _2 X$ e6 D |
1301
- E. e" R5 \: [! Q* G | hptII 6 c$ L1 b# {# V5 H2 v- {
| kan
/ [# f( W6 V/ d | pUC18
3 C& d, N! P$ l- {8 q; \% |- L8 D | gusA + r( q' ~1 x- R) X6 k9 |
| 56074 T% r2 ~- N& r. N3 R
| -/ B9 R9 ^: f/ p5 e+ v
| GIS* q) h$ ]; G4 {' D: O! }: V1 Y/ t: J
| view map
: E: n: s$ Z& y' E \ |
1300 1 I V- O/ ]/ ` p+ A6 n& ?
| hptII
E5 y7 q) @: k7 M# k/ w | kan
4 ]! ^6 c; X/ h, _ | pUC18- a/ d# h( C5 `! @5 j
| -7 l$ [( i( B7 Z" v6 T
| 2728$ J& i3 G/ Z5 { u) t9 l# u+ C' \
| -
, O* J, A2 t8 W0 Y | MSV
" C6 r8 M1 U. G | view map
/ ^, D1 u3 c+ p" U |
1291Z
2 h6 w- h+ ^* u, u$ E! q. d | hptII * n7 `/ ~1 _ g$ R
| cmr2 D9 \4 R1 \' R* \
| pUC9
$ o% P5 u3 R) ?; F* i- l5 c | gusA
! m6 Z! d d u& S: T | 4984+ z! Y+ H! e2 U* E; w; s
| lacZ 6 \0 i- g/ M' H9 j q& e6 O7 y: f
| PCV
0 m* g0 H1 k% k: p( { | view map B% v; i- ]2 P6 |( p0 i5 U
|
1281Z & i1 G& M1 Y) c+ J: Y
| hptII $ G0 W: t" }& V. J
| cmr
, v9 n! N7 X) Y& \0 ` | pUC8
( u% l! B3 P' N0 A: h | gusA " \. j9 c2 z- ~/ W9 ^4 h' {
| 49843 p2 }2 L5 J8 b/ G
| lacZ
h3 S# q, Y" E | PCV
! U# e- m/ c& [ z* _8 v | view map + |* e, V) L* Z+ p. N
|
1201
1 Q) a5 J4 T3 A" M1 h | hptII
M b& K' S- A+ _ g/ K | cmr3 @8 U7 f1 C# R/ j; _, e
| pUC18$ ~! t5 S) ^6 K* z3 i7 f& N
| gusA ! H/ v0 D5 h/ l; C0 |; T8 H( i- |
| 5606& P4 E' V' O- q' Q) `3 I
| -
$ `% ]5 O* P, b# C2 ]" o+ j1 t, X | GIS
) \5 L; R4 X3 m! `" H+ Z! m. U/ I | view map
& k& L0 k- p+ C7 S h |
1200 + b/ }' N$ h! P" b( C$ ?" C. s
| hptII
0 U& ~; B; V. v | cmr# P; ^- M- F. |) V' T
| pUC183 v- M" P; w/ S: ~3 ]1 G
| -; M- N$ X: i f' F3 ?5 M
| 2727
% V y8 B! w3 w1 Q5 k& u: c; K* p | -
: t; y' \4 i' T, N6 w# F | MSV
4 v, {7 V3 }/ Y( f) N0 B v9 G | view map & {4 F; ~4 N& d: b6 H
|
0390
! n+ C& O4 Y7 c$ s0 d | -9 h( {9 g4 \. o! F
| kan
; w+ z/ K7 S0 A% `+ ^$ _, Y. i+ X | pUC9. e* e7 D4 e% `! f( [
| -
' k4 Z9 h8 i! K' r+ E( y4 W | 582, F3 z: g" j9 N1 {
| -
) @4 e0 U# k, b8 L | DIY, ?( s( L4 @$ F1 y2 Z3 ]
| view map 4 @9 Q/ j0 j% N0 M4 F) E. S
|
0380 / n6 S8 e Z$ {
| -, Y( T9 j2 L: U# [
| kan9 w1 M1 Y7 M# H+ n9 P
| pUC8
9 K& u7 u) @/ k( c | -
, J" H5 j2 G$ a | 582; N8 W% @) _# b. U
| -6 S: Q; O8 U" c7 e: C
| DIY
/ d% E3 D Z( L& t( |+ S. m4 s; Y4 F | view ma
1 }" U1 u+ Q+ C- P( E% i- _) K5 ] |
3300 bar kan 2195 LacZ
3301 bar kan gusA 5034 LacZ
